Preparation and Physicochemical Evaluation of Hydroxyapatite-gelatin Nanoparticles

One of the promising fields research is tissue engineering that can facilitate available therapeutic methods. Nanosized hydroxyapatite (HA) one key elements in mineral bone. It possible to affect surrounding osteoprogenitor cells using nano-sized HA and through this improve bone repair changing paracrine signaling. The present paper an attempt prepare evaluate physicochemical properties hydroxyapatite-gelatin nanoparticles. To end, two sizes were prepared including S100 S150 standard chemical precipitation. characterize size morphology synthesized powders, X-ray diffraction Brunauer-Emmett-Teller (BET) surface area determined Autosrob-IQ2-MP. measure calcium ions released by HANPs, inductively coupled plasma optical emission spectrometer (ICP-OES) was used. collected data analyzed SPSS 19.0. In case S100, hydrodynamic diameter based on DLS analyses equal 626.10±14.95nm; figure for 262.33±46.5. There a larger specific compared S150; addition, had wider peaks, which agreement with small poorly crystalline crystals. On other hand, peaks sharper, means crystallinity higher S150. HANPs all degradability smaller (S100) degraded faster larger-sized pH level control, 7.24±0.01, 7.26±0.02 so there no significant difference between them. Nanoparticle factor biological environment, provides reference biomedical uses.